Hands-on bioinformatics demos

Two guided analyses on public data. Pick samples, run each step, change the parameters and see what moves. Heavy steps were computed ahead of time; the rest runs live. Works on a phone.

16S amplicon

Oral microbiome, reads to species

FASTQ, primer trimming and MultiQC, DADA2 with three parameter sets, taxonomy, alpha and beta diversity, and differential abundance with MaAsLin 3 on your own selection.

Public study SRP562347 · 81 plaque samples

Open the 16S demo →
Single-cell RNA-seq

Oral cancer, count matrix to pathways

Cell filtering, dimension reduction and UMAP, Leiden clustering at three resolutions, cell-type checks with markers and CellTypist, then pseudobulk DESeq2 and GSEA.

Public study GSE181919 · 17 oral-cavity samples, 24,156 cells

Open the scRNA-seq demo →